DMA Damage Induced in Human Diploid Cells by Decay of Incorporated Radionuclides1

نویسندگان

  • Peter K. LeMotte
  • John B. Little
چکیده

Alkaline and neutral elution techniques were used to charac terize the production of singleand double-strand DMA breaks in human diploid fibroblasts by incorporated radionuclides. 125I was incorporated in DMA as [125l]iododeoxyuridine, 3H as [3H]thymidine, and 14C as [14C]thymidine. Under frozen conditions, 125Iwas 3 times as efficient as 3H per decay in inducing singlestrand breaks and 6 times as efficient as 14C.For double-strand break production, however, 125Iwas 6 times as efficient per decay as 3H. It was calculated that, on the average, each 125I decay produces about one double-strand break in the frozen state. Under nonfrozen conditions, 125Iand external X-rays were roughly 5-fold and 3H about 3-fold more efficient in double-strand break induction than under frozen conditions.

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تاریخ انتشار 2006